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Specifications
- Antibody type:Primary
- Antigen name:nicotinamide phosphoribosyltransferase
- Clonality:Polyclonal
- Gene ID:P43490
- Host:Rabbit
- Reactivity:Human
- Antigen symbol:NAMPT
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- Size:100 ug
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:NAMPT
- Storage buffer:Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
- Storage temperature:Stable for at least 6 months after receipt when stored at –20 °C.
- Concentration:1 mg/mL
- Shipping temperature:4 °C
- Immunogen:Synthetic peptide corresponding to aa 413-431 (V413FKDPVADPNKRSKKGRLS431) of human Nampt (Visfatin/PBEF).
- Cat. no.:75793-312
- Supplier No.:36-226
Specifications
About this item
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (paraffin sections, 10ug/ml). Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Type: Primary
Antigen: NAMPT
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human