To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Antibody type:Primary
- Antigen name:chemokine (C-C motif) ligand 3
- Clonality:Monoclonal
- Clone:1.2_3E8-2H6-2B6
- Gene ID:P10147
- Host:Mouse
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:CCL3
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.5 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:CCL3
- Storage temperature:Stable at room temperature for at least one month and for greater than a year when kept at –20 °C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. Stable for at least six weeks at 2-4 °C when reconstituted.
- Shipping temperature:RT
- Immunogen:The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1α as the immunizing antigen.
- Purification:This IgG1 antibody was purified from ascites fluid by SAS precipitation followed by ion exchange column.
- Cat. no.:10816-214
- Supplier No.:XP-5734-M
Specifications
About this item
Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1alpha (100 ng/mL), a concentration of 5.0-10.0 ug/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 uL/well), a concentration of 2.0-4.0 ug/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1alpha when used with our biotinylated antigen affinity purified anti-human MIP-1alpha (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 ug/mL. Western Blot: To detect hMIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1alpha is 1.0-2.0 ng/lane, under non-reducing conditions.
Type: Primary
Antigen: CCL3
Clonality: Monoclonal
Clone: 1.2_3E8-2H6-2B6
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human