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Specifications
- Antibody type:Primary
- Antigen name:interleukin 7
- Clonality:Monoclonal
- Clone:NYR-hi7
- Gene ID:P13232
- Host:Mouse
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:IL7
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.5 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:IL7
- Storage temperature:Stable at room temperature for at least one month and for greater than a year when kept at –20 °C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. Stable for at least six weeks at 2-4 °C when reconstituted.
- Shipping temperature:RT
- Immunogen:The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-7 as the immunizing antigen.
- Purification:This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.
- Cat. no.:10813-640
- Supplier No.:XP-5705-M
Specifications
About this item
Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL-2, IL-4 and IL-6, suggesting that IL-7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. In myeloid lineage cells, IL-7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL 7 (0.5 ng/mL), a concentration of 0.05-0.08 ug/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 uL/well), a concentration of 2.0-4.0 ug/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with our biotinylated antigen affinity purified anti-human IL-7 (XP-5197Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/mL. Western Blot: To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Type: Primary
Antigen: IL7
Clonality: Monoclonal
Clone: NYR-hi7
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human