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Specifications
- Conjugate ELISA:HRP-Streptavidin
- Target protein:TNF-alpha
- Size:480 assays
- Cat. no.:CAPIESS0017
- Supplier No.:ESS0017
Specifications
About this item
Assay TNFα (equine tumor necrosis factor alpha, TNF alpha) levels in horse cell culture supernatant samples with our Thermo Scientific Equine TNF alpha ELISA Kits.
This equine TNF alpha ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of horse tumor necrosis factor alpha (TNFα) in samples at 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. This economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
Features of the Equine TNF alpha ELISA Reagent Kit:
Target: natural and recombinant horse TNFα (equine tumor necrosis factor alpha)
Format: colorimetric ELISA (TMB substrate) for 96-well microplates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 16 to 1000 pg/mL
Sensitivity: undetermined
Sample Types: cell culture supernatant
Total Assay Time: 3 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with bovine, mouse, or rat TNFα. Partial cross-reaction occurs with human and porcine TNFα (< 10%).
Includes
The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.