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Specifications
- Reactivity:Human
- Target protein:IL-6
- Size:96 assays
- Cat. no.:CAPIEH2IL6
- Supplier No.:EH2IL6
Specifications
About this item
The Thermo Scientific Human IL-6 ELISA Kit is used to measure IL-6 (human interleukin-6) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatants. Each kit contains one 96-well Strip Plate precoated with a human IL-6 capture antibody and sufficient detection antibody, conjugate, buffers, diluent, TMB and stop buffers, as well as lyophilized human IL-6 standard to analyze several samples.
Features of the Thermo Scientific Human IL-6 ELISA Kit: †
Target: natural and recombinant human interleukin-6 (IL-6)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 10.2 to 400 pg/mL
Sensitivity: < 1 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFα, IFNγ, IFNα, GM-CSF, or mouse IL-6.
† Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.
These human IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-6 antibody pairs and IL-6 standard only. All components undergo quality control testing to minimize lot-to-lot variability.