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Specifications
- Description:Micrococcal Nuclease Solution (≥100 U/µL)
- Size:150 µL
- Cat. no.:CAPI88216
- Supplier No.:88216
Specifications
About this item
The Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
- Enzyme digests nucleic acids (DNA and RNA)
- Effective for double-stranded, single-stranded, circular and linear nucleic acids
- Active in neutral to alkaline buffer conditions containing divalent calcium ions
- Supplied at ≥100 units/µL in 10 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM EDTA, 50% glycerol
Enzyme is active at pH 7 to 10 with a salt concentration less than 100 mM
This micrococcal nuclease is a stable liquid form of the enzyme derived from Staphylococcus aureus. Micrococcal nuclease (MNase) exhibits exo- and endo-5'-phosphodiesterase activities against DNA and RNA. This enzyme digests double-stranded, single-stranded, circular and linear nucleic acids. The highest activity is toward single-stranded nucleic acid substrates with preference for AT- or AU-rich regions. Enzymatic activity occurs at pH 7 to 10 and is strictly dependent on calcium for digestion of RNA and DNA substrates. Micrococcal Nuclease is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
Optimal enzyme activity occurs at 37˚C; however, the enzyme is active at room temperature. Monovalent metal ions, such as Na+ and K+, will decrease activity. EGTA or heating to 65˚C for 10 minutes will inactivate the enzyme.