The Melon Gel Spin Plate Kit for IgG Screening offers a high-throughput format for quick purification and screening of antibodies.

• Simple, one-step protocol with rapid purification
• Minimal dilution, reduced need to concentrate antibody before storage or use
• Gentle purification, no harsh antibody elution conditions
• Melon™ gel resin can be used for multiple antibody purifications

This kit enables purification of up to 96 samples of 20-100 µl in 15 minutes or less, offering a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Melon Gel IgG Purification Kits enable purification of antibodies from serum and other sample types. Melon Gel Resin binds and removes serum proteins, thereby allowing pure IgG to be collected in the flow-through fraction. The procedure is gentle because no harsh elution conditions are involved.

Melon Gel Resin contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because Melon Gel Resin is not a bind-and-release support, it is extremely fast and gentle to ant ibodies, resulting in antibody preparations of high purity and high activity.

Melon Gel Resin works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with Melon Gel Resin are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.