Pierce™ AminoLink™ Plus Immobilization Kit, 2 ml, Thermo Scientific
Supplier: Thermo Scientific
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About this item
The AminoLink Plus Immobilization Kit contains AminoLink Plus Coupling Resin and additional reagents needed to perform 5 protein immobilizations, with expected yields of 2 to 40 mg protein per column.
- Aldehyde-activated crosslinked 4% beaded agarose
- Immobilize molecules via primary amines (-NH2)
- Efficient coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
- Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
- Versatile and reusable
The coupling resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.
Delivery: Kit contains AminoLink™ Plus columns, 2 mL (5 columns), PBS pack (makes 500 mL), citrate-carbonate buffer pack (makes 500 mL), sodium cyanoborohydride solution, 5 M (0.5 mL), quenching buffer (60 mL), wash solution (240 mL), and column accessories.
Caution: For Research Use Only. Not for use in diagnostic procedures.
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