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Specifications
- Description:Pierce™ F(ab)2 Micro Preparation Kit
- Size:1 kit
- Storage conditions:Sufficient For: 10 antibody samples, each containing 25 to 250 µg IgG
Pierce Pepsin Agarose, 0.5 mL
F(ab')2 Digestion Buffer, 55 mL
NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns
PBS Packs (each makes 500 mL), 2 packs
IgG Elution Buffer, 50 mL
Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns
Spin Columns, 0.8 mL, 10 columns
Microcentrifuge Tubes, 2 mL, 30 tubes
Store at 4˚C - Cat. no.:CAPI44688
- Supplier No.:44688
- Sufficient For:Sufficient for 10 antibody samples, each containing 25 - 250 µg IgG
Specifications
About this item
Pierce F(ab')2 Micro Preparation Kit uses immobilized pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.
- Enzyme free digestion products
- Enhanced yield and sample purity
- Spin format reduces sample processing time
- Ideal when sample size is limited—Use the micro kit for 25 to 250 µg IgG.
- Flexible, protocols are included for multiple species and IgG subclasses, as well as sample size and concentration
Suitable for human and other species of IgG. The kit procedure is optimized for human, mouse and rabbit IgG. Papain-based digestion is effective for certain other species and subclasses of IgG (e.g., goat and pig), although the purification step requires that the antibody effectively binds to Protein A. Immobilized pepsin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme.