About this item
Thermo Scientific Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
- Purified form—lyophilized salt-free powder; ready to dissolve and use
- High specific activity—typically greater than 300 units/mg (lot-specific value reported)
- Compared to AP—HRP is smaller (40kDa) than alkaline phosphatase (AP; 140kDa) and has higher specific enzyme activity than both AP and beta-galactosidase (b-Gal)
- Many options—numerous substrate solutions and assay techniques are available for HRP
This purified horseradish peroxidase (HRP) is supplied lyophilized as a salt-free powder for reconstitution and use in protein research methods
One unit catalyzes the production of 1 mg of purpurogallin from pyrogallol in 20 seconds at 20˚C and pH 6.0.
The main application for HRP in molecular biology and protein research is as a reporter system for immunoassays and other probe-based assay techniques such as ELISA, Western blotting, EMSA and Southern blotting. The enzyme is usually conjugated to specific secondary antibodies or streptavidin, and its activity is detected with a color-forming (or light-generating) substrate.
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