These NeutrAvidin™ Agarose is a beaded agarose resin of immobilized NeutrAvidin™ Protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

• Tetrameric with four biotin-binding site per molecule
• Support is crosslinked 6% beaded agarose (CL-6B)
• Immobilised by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding

NeutrAvidin™ agarose consists of a specially purified and deglycosylated form of avidin used for variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Standard capacity beads: Have a normal load of immobilised NeutrAvidin™ protein, providing a binding capacity of 1 to 2 mg of biotinylated BSA per ml of resin.

High capacity beads: Have a dense load of immobilised NeutrAvidin™ protein, providing a binding capacity greater than 8 mg of biotinylated BSA per ml of resin.

Properties of crosslinked 6% beaded agarose (CL-6B):
Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
Average Particle Size: 45 to 165 microns
Exclusion Limit: 10,000 to 4,000,000 daltons
Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
Maximum Linear Velocity: 30 cm per hour
Maximum Pressure: less than 25psi (1.5 bar)