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Specifications
- Description:TNBSA solution (2,4,6-trinitrobenzene sulfonic acid), 5% w/v in methanol
- Size:100 mL
- Cat. no.:CAPI28997
- Supplier No.:28997
Specifications
About this item
Thermo Scientific Pierce TNBSA Solution is a 5% solution of trinitrobenzene sulfonic acid in methanol that reacts with primary amines (peptides or amino acids) to yield a soluble colored product, a property useful for various assay methods.
- TNBSA component reacts readily with primary amino groups of amino acids in aqueous format at pH 8 to form yellow adducts
- Coloured derivatives are monitored at 345 nm and have extinction coefficients of 10,000 to 15,000
- No coloured derivatives are formed with secondary amino acids proline and hydroxyproline
- Applicable to solution or solid phase analysis
TNBSA is used as a hydrophilic modifying reagent for the detection of primary amines in samples containing amino acids, peptides or proteins. It is an excellent reagent for rapid qualitative and quantitative estimation of these biomolecules.
TNBSA is used as a hydrophilic modifying reagent for the detection of primary amines in samples containing amino acids, peptides or proteins. It is an excellent reagent for rapid qualitative and quantitative estimation of these biomolecules.