Pierce™ Immunoprecipitation, Magnetic ChIP Kit, Thermo Scientific

Catalog #: CAPI26157

Supplier: Thermo Scientific

CAS Number:

SDS

Pierce™ Immunoprecipitation, Magnetic ChIP Kit, Thermo Scientific

Catalog #: CAPI26157

Supplier: Thermo Scientific

CAS Number:

SDS

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Specifications

Description:
Magnetic ChIP Kit
Size:
30 reactions
Storage temperature:
-20 °C to 4 °C
Cat. no.:
CAPI26157
Supplier No.:
26157
Sufficient For:
30 ChIP assays

Specifications

About this item

The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.

Purified DNA for quantitative PCR can be obtained in about 8 hours
Optimized micrococcal nuclease digestion and nuclear lysis
Obtain results with as few as 10,000 cells
Pierce protein A/G magnetic beads are blocked in a non-DNA-containing reagent to minimize background

The blocked protein A/G magnetic beads provide high binding capacity, low non specific background, and work with many antibody species. These beads can be used manually with a magnetic stand or with automated platforms.

Ordering information: For the effective performance, In vivo crosslinker (such as formaldehyde), micro-tip sonicator (such as Misonix™ Sonicator 3000), ChIP qualified antibody of choice, PCR primers for DNA sequence of interest, PCR master mix (containing a dye such as SYBR™ Green, if qPCR is desired), and a qPCR instrument are necessary.

Delivery: Kit includes Glycine Solution, 10X, (15 mL), PBS, 20X, (15 mL), Halt™ Protease and Phosphatase Inhibitor Cocktail (EDTA-free), 100X, (1 mL), Membrane Extraction Buffer (10 mL), MNase Digestion Buffer (10 mL), DTT (7.7 mg), Lyophilized (2 vials), MNase Stop Solution (1 mL), ChIP-Grade Protein A/G Magnetic Beads (0.7 mL), IP Dilution/Wash Buffer, 5X, (40 mL), Sodium Chloride, 5M, (6 mL), IP Elution Buffer, 2X, (4.5 mL), Microcentrifuge Tubes (1.5 mL), 75 tubes, DNA Clean-Up Column and Reagents (40 purifications), DNA Clean-Up Column (40 columns), DNA Column Binding Solution (30 mL), DNA Column Wash Solution (6 mL), pH Indicator (0.8 mL), DNA Column Elution Solution (5 mL), Micrococcal Nuclease (ChIP Grade), 10U/µL, (50 µL), Anti-RNA Polymerase II Antibody (25 µL), Normal Rabbit IgG, 1mg/mL, (10 µL), Proteinase K, 20mg/mL, (0.25 mL), ChIP Positive Control Primers, GAPDH promoter, Human-specific, (100 µL).

Caution: For Research Use Only. Not for use in diagnostic procedures.

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