Pierce™ Agarose ChIP Kit, Thermo Scientific

Catalog #: CAPI26156

Supplier: Thermo Scientific

CAS Number:

SDS

Certificates

Pierce™ Agarose ChIP Kit, Thermo Scientific

Catalog #: CAPI26156

Supplier: Thermo Scientific

CAS Number:

SDS

Certificates

Specifications

Description:
Agarose ChIP Kit
Cat. no.:
CAPI26156
Supplier No.:
26156
Sufficient For:
Performing ChIP Reactions for 30 Target Proteins from 10 Sample Conditions

Specifications

About this item

The Thermo Scientific Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

Simple and fast ChIP assay protocol (< 8 hours)
Highly efficient isolation and lysis of nuclei
Easy and reproducible enzymatic digestion
Low-background and high-binding capacity Protein A/G agarose resin
Highly specific positive controls included: RNA polymerase II antibody and GAPDH PCR primers
Fast and reproducible spin-column format
High-recovery DNA purification
ChIP-validated antibodies available

ChIP assay has the ability to capture a snapshot of specific protein-DNA interactions as they occur in living cells, and then quantitate the interactions using standard or quantitive PCR.

Delivery: Kit includes ChIP Grade Protein A/G Plus Agarose (0.65 mL), IP Dilution/Wash Buffer, 5X, (11 mL), IP Wash Buffer 3, 5X, (4.5 mL), IP Elution Buffer, 2X, (4.5 mL), Column Accessory Pack (3 packs), Microcentrifuge Tubes (1.5mL), 75 tubes, DNA Clean-Up Column (40 columns), DNA Column Binding Solution (30 mL), DNA Column Wash Solution (6 mL), pH Indicator (0.8 mL), DNA Column Elution Solution (5 mL), Anti-RNA Polymerase II Antibody (25 µL), Normal Rabbit IgG, 1 mg/mL, (10 µL), ChIP Positive Control Primers, GAPDH promoter, Human-specific, (100 µL), Pierce Chromatin Prep Module.

Caution: For Research Use Only. Not for use in diagnostic procedures.

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