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Specifications
- Description:Direct IP Kit
- Size:50 reactions
- Storage temperature:4 °C
- Supplier No.:26148
- Sufficient For:50 Reactions using 10 μl of immobilized antibody support
Specifications
About this item
The Pierce™ Direct IP Kit uses an activated resin to covalently immobilize IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.
- Requires less than 10 µg of antibody for a single IP experiment
- Eliminates antibody contamination
- Immobilize 100 to 200 µg of antibody to prepare ready-made IP affinity resin
- Spin columns eliminate resin loss and provide for more efficient separation of solutions
- Use chicken IgY, human IgE, mouse IgM or any other purified protein
The primary benefits resulting from this method are the opportunity to use any species or subclass of purified antibody (not just types that bind to Protein A or G) and the ability to purify target protein without contamination by the antibody. The method also makes it possible to immunoprecipitate antigens from serum samples without co-purifying non-target immunoglobulins. Finally, the kit uses microcentrifuge spin cups to effectively wash and separate samples from the beaded agarose resin.