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Specifications
- Description:Crosslink IP Kit. Sufficient for 50 Reactions using 10 μL of Immobilized Antibody Support
- Size:50 reactions
- Storage temperature:4 °C
- Cat. no.:CAPI26147
- Supplier No.:26147
Specifications
About this item
The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.
- Eliminates antibody contamination
- Immobilize 100 - 200 µg of antibody to prepare ready-made immunoprecipitation affinity resin (reusable) for many experiments
- Spin columns eliminate resin loss and provide for more efficient separation of solutions
The primary benefits resulting from these features are the ability to purify target protein without contamination by the antibody and the ability to more effectively wash and separate samples from the beaded agarose resin. This IP Kit method involves capturing the IP antibody to Protein A/G Agarose resin and covalently immobilizing it to the support by crosslinking with disuccinmidyl suberate (DSS).