Thermo Scientific AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

• Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH₂)
• Efficient (>85%) coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer, with or without organic solvent)
• Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
• Better than immobilization to CNBr-activated agarose as the bond is more stable and uncharged, resulting in less non specific binding in affinity purification procedures
• Prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

The AminoLink Resin immobilization reaction involves spontaneous formation of Schiff base bonds between aldehydes and amines and their subsequent stabilization by incubation with a mild reductant.