Thermo Scientific Pierce CDI-Activated Agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilization of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

• No secondary reagents needed since rection proceeds spontaneously
• Half-life of hydrolysis is much longer than hydroxysuccinimide ester activations
• Reaction is most efficient with primary amines at pH 9 to 11
• Immobilization occurs through the reaction of N-nucleophiles with 1,1' -carbonyldiimidazole groups of the resin to form a stable, uncharged N-alkylcarbamate linkages
• Available in convenient form as it is supplied as stabilized, 50% slurry in acetone

Pierce CDI-activated Resin is porous, beaded agarose chromatography resin that is activated with carbonyl diimidazole groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles. Coupling can be performed in aqueous or organic solvent conditions, making possible the immobilization of many kinds of ligands. Once a molecule is immobilized, the prepared affinity resin can be used multiple times in typical affinity purification procedures.