Thermo Scientific Pierce Iminobiotin Agarose provides for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components.

• Crosslinked beaded agarose onto which iminobiotin is covalently immobilized
• Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)

Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Because no denaturing agents such as 8 M guanidine hydrochloride or 4 M urea are required for elution, the avidin conjugate has a better chance of maintaining its activity after purification. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.