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Specifications
- Description:HisProbe™-HRP conjugate, Lyophilized powder
- Size:2 mg
- Storage conditions:Upon receipt store product at 4˚C.
- Shipping temperature:ambient
- Cat. no.:CAPI15165
- Supplier No.:15165
Specifications
About this item
Thermo Scientific HisProbe-HRP is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
- One-step probe incubation eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
- Recognize polyhistidine tags independent of adjacent tags
- Smaller than antibodies and detects his tagged fusion proteins that are often undetectable
Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC). The Thermo Scientific SuperSignal West HisProbe Kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate.
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