G-Biosciences' GenLysate™ total protein lysates are ready to use for SDS-PAGE and Western blotting purposes.

• Extracted from the tissue or cell of interest
• Ready for use
• Available in a wide variety of different species and tissues

G-Biosciences' GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest

The total proteins are being extracted from whole tissue or cells following the method of Laemmli. The proteins are extracted in a denaturing buffer containing 62 mM Tris HCl and 2% SDS with G-Biosciences Protease ARREST™ protease inhibitor cocktail. This minimizes proteolytic damage to the proteins.

G-Biosciences GenLysate™ total protein lysates are offered as a 150 µg lyophilized protein per vial and, following reconstitution in DI water, are ready for use. G-Biosciences offers GenLysate™ in a wide variety of different species and tissues. Species include: human (both normal tissue and tumor tissue lysates available), mouse, rat, macaque (primate), rabbit, pig, plant-related, bacteria, insect, bird, frog, fish, marsupial, earthworm, and yeast.

To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail.