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Specifications
- Antibody type:Primary
- Antigen name:Phosphatidylinositol 4-kinase Type 2 Alpha
- ChIP:Yes
- Clonality:Polyclonal
- Gel shift:Yes
- Gene ID:PI4K2A/55361; Pi4k2a/84095
- Host:Rabbit
- Isotype:IgG
- Neutralisation:Yes
- Reactivity:Human,Mouse
- Antigen symbol:PI4K2A
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:400 µL
- Western blot:Yes
- Cross adsorption:No
- Form:Liquid
- Antigen synonyms:PIK42A|Phosphatidylinositol 4-kinase type II|PI4KII|Type II alpha phosphatidylinositol 4-kinase
- Storage buffer:PBS with 0.09% Sodium azide
- Molecular weight:54.02 kDa
- Storage temperature:Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at –20 °C
- Concentration:Lot-specific
- Immunogen:KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI4K2A (PI4K II)
- Tested applications:Immunohistochemistry
- Purification:Ammonium sulfate precipitation, Size-exclusion - Dialysis
- Cat. no.:CAPIPA5-15275
- Supplier No.:PA515275
Specifications
About this item
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs. PI4K II is activated by detergent and inhibited by adenosine. Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P. Some cells overexpressing PI4KII have scattered or no perinuclear Golgi. Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi. RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network. PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35%. It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.
Recommended Dilutions: Western Blot: 1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry: 1:50-1:100
Type: Primary
Antigen: PI4K2A (phosphatidylinositol 4-kinase type 2 alpha)
Clonality: polyclonal
Clone:
Conjugation: unconjugated
Epitope:
Host: Rabbit
Isotype: none
Reactivity: Human