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Specifications
- Antibody type:Primary
- Antigen name:Microtubule-associated Protein 1 light chain 3 beta
- ChIP:Yes
- Clonality:Polyclonal
- Gel shift:Yes
- Host:Rabbit
- Isotype:IgG
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:LC3B
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:400 µL
- Western blot:Yes
- Cross adsorption:No
- Form:Liquid
- Antigen synonyms:MAP1LC3B|Autophagy-related protein LC3 B|MAP1 light chain 3-like protein 2|MAP1ALC3|MAP1A/MAP1B LC3 B|Microtubule-associated proteins 1A/1B light chain 3B [Precursor]|MAP1A/1B light chain 3 B|Autophagy-related ubiquitin-like modifier LC3 B
- Modification:phospho-Thr12
- Storage buffer:PBS with 0.09% Sodium azide
- Molecular weight:14.56 kDa
- Storage temperature:Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at –20 °C
- Concentration:Lot-specific
- Immunogen:KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T12 of human LC3B
- Tested applications:Dot Blot
- Purification:Antigen affinity chromatography
- Cat. no.:CAPIPA5-12959
- Supplier No.:PA512959
Specifications
About this item
This antibody is predicted to react with bovine based on sequence homology. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
Recommended Dilutions: Dot blot: 1:500
Type: Primary
Antigen: MAP1LC3A (microtubule-associated protein 1 light chain 3 alpha)
Clonality: polyclonal
Clone:
Conjugation: unconjugated
Epitope:
Host: Rabbit
Isotype: none
Reactivity: Human