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Specifications
- Antibody type:Primary
- Antigen name:Heat Shock Protein 27
- ChIP:Yes
- Clonality:Polyclonal
- Gel shift:Yes
- Host:Rabbit
- Isotype:IgG
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:HSP27
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:400 µL
- Western blot:Yes
- Cross adsorption:No
- Form:Liquid
- Antigen synonyms:SRP27|HSP27|HSP28|HspB1|Hsp25|Hs.76067|HSPB1|HS.76067|DKFZp586P1322|HMN2B|CMT2F
- Modification:phospho-Ser78
- Storage buffer:PBS with 0.09% Sodium azide
- Molecular weight:22.78 kDa
- Storage temperature:Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at –20 °C
- Concentration:Lot-specific
- Immunogen:KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S78 of human HSPB1
- Tested applications:Dot Blot
- Purification:Antigen affinity chromatography
- Cat. no.:CAPIPA5-12779
- Supplier No.:PA512779
Specifications
About this item
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.
Recommended Dilutions: Dot blot: 1:500
Type: Primary
Antigen: HSPB1 (heat shock 27kDa protein 1)
Clonality: polyclonal
Clone:
Conjugation: unconjugated
Epitope:
Host: Rabbit
Isotype: none
Reactivity: Human