Anti-APEX1 Rabbit Polyclonal Antibody

Catalog #: 10409-570

Supplier: Bioss

Catalog #: 10409-570

Supplier: Bioss

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Specifications

Antibody type:
Primary
Antigen name:
APEX Nuclease (Multifunctional DNA Repair Enzyme) 1
Clonality:
Polyclonal
Gene ID:
328
Host:
Rabbit
Isotype:
IgG
Reactivity:
Human,
Rat,
Mouse
Antigen symbol:
APEX1
Conjugation:
Unconjugated
ImmunoChemistry:
Yes
Size:
100 µg
Western blot:
Yes
Cross adsorption:
No
Form:
liquid
Antigen synonyms:
Protein REF-1|APEX|DNA-(apurinic or apyrimidinic site) lyase|APEN|REF1|APE|APEX nuclease|APX|AP endonuclease 1|Apurinic-apyrimidinic endonuclease 1|APEX1|HAP1
Storage buffer:
Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Storage temperature:
Store at –20 °C for 12 months
Concentration:
1 μg/μl
Shipping temperature:
4°C
Immunogen:
1450-1500/1870
Purification:
Purified by Protein A
Cat. no.:
10409-570
Supplier No.:
BS-5150R

Specifications

About this item

Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs).