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Specifications
- Antibody type:Primary
- Antigen name:Caspase 3
- Clonality:Polyclonal
- Gene ID:836
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Antigen symbol:CASP3
- Conjugation:Alexa Fluor® 488
- ImmunoChemistry:Yes
- Size:100 µL
- Cross adsorption:No
- Form:liquid
- Antigen synonyms:Caspase-3 subunit p12|Protein Yama|Cysteine protease CPP32|3.4.22.56|CASP-3|Cysteine protease CPP32 Caspase-3|Apopain|Caspase-3 subunit p17|Cpp32|SCA-1|CPP-32|SREBP cleavage activity 1
- Storage buffer:Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
- Storage temperature:Store at 4°C for 12 months
- Concentration:1 μg/μl
- Shipping temperature:4°C
- Purification:Purified by Protein A
- Cat. no.:10230-880
- Supplier No.:BS-0087R-A488
Specifications
About this item
The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Recommended Dilutions: IF(IHC-P): 1:50-200
Type: Primary
Antigen: CASP3
Clonality: Polyclonal
Clone:
Conjugation: Alexa Fluor® 488
Epitope:
Host: Rabbit
Isotype: IgG1
Reactivity: Human, Mouse, Rat