Glutathione Sepharose™ 4 Fast Flow Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Certificates
About this item
Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.
- For affinity chromatography
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose 4 Fast Flow via a 10-carbon spacer arm
- Epoxy activation coupling method
- Stable in all commonly used aqueous buffers
Mild elution conditions preserve protein antigenicity and function. Sepharose 4 Fast Flow provides good flow properties and is excellent for scaling up. Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Related Information
Certificates
Search by Lot NumberCytiva CyDye Licensing Statements Cytiva General Licensing Statements
Additional Product Details
Customers Who Bought This Also Bought