Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

• For affinity chromatography
• BioProcess medium supported for industrial applications and well-established in approved processes
• Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity
• Triazine coupling method
• Stable in commonly used aqueous buffers - 70% ethanol, 6 M guanidine hydrochloric, 8 M urea

Blue Sepharose 6 Fast Flow has been developed and tested in co-operation with leading large scale manufacturers and is used in routine commercial production

Blue Sepharose 6 Fast Flow is a member of the Cytiva (formerly GE Healthcare Life Sciences) range of affinity chromatography media for capture and intermediate purification. It is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

Blue Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Blue Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin