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Specifications
- Antibody type:Primary
- Clonality:Monoclonal
- Clone:9C8
- Gene ID:DDIT3/1649; Ddit3/13198
- Host:Mouse
- Isotype:IgG2b
- Reactivity:Human,Mouse
- Antigen symbol:CHOP
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:100 µg
- Western blot:Yes
- Cross adsorption:No
- Antigen synonyms:C/EBP Homology Protein|GADD153|Growth Arrest and DNA Damage Inducible Protein 153
- Storage buffer:PBS with 1mg/ml BSA and 0.05% Sodium azide
- Storage temperature:–20 °C, avoid freeze/thaw cycles
- Tested applications:Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
- Cat. no.:CAPIMA1250
- Supplier No.:MA1250
Specifications
About this item
MA1-250 detects CHOP protein in human and mouse samples. MA1-250 has successfully been used in Western blot, immunoprecipitation, immunocytochemistry, and immunofluorescence procedures. By Western blot, this antibody detects an ~31 kDa protein representing CHOP from primary human fibroblast extract. The MA1-250 immunizing protein corresponds to a bacterially expressed, mouse CHOP fusion protein. Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP. This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results. CHOP (also known as GADD153, Growth Arrest and DNA Damage Inducible Protein 153, and C/EBP Homology Protein) is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly upregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.
Recommended Dilutions: Western Blot: 1 ug/ml, Immunofluorescence: Assay dependent, Immunocytochemistry: Assay dependent, Immunohistochemistry (Frozen): 1/100, Immunohistochemistry (Paraffin): Assay Dependent, Immunoprecipitation: Assay dependent
Type: Primary
Antigen: CHOP
Clonality: Monoclonal
Clone: 9C8
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Human, Mouse