About this item
NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature.
- Highly specific, thereby minimizes the non-specific amplifications
- Improved low-copy target amplification
- Ambient temperature setup compatible with automation
- Target amplification of up to 5 kbp
- Ideal for quantitative and high-throughput PCR applications
The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products.
The enzyme must be activated by heat treatment (7 - 10 min at 95 °C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-nucleotide overhangs, suitable for cloning with a varierty of vector kits.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.