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Specifications
- Antibody type:Primary
- Antigen name:HLA class II histocompatibility antigen, DR alpha chain
- Clonality:Monoclonal
- Clone:MEM-12
- Gel shift:Yes
- Host:Mouse
- Isotype:IgG1
- Reactivity:Human
- Antigen symbol:HLA-DR
- Conjugation:PerCP (Peridinin-Chlorophyll Protein Complex)
- Flow cytometry:Yes
- ImmunoPrecipitation:Yes
- Size:100 test
- Western blot:Yes
- Antigen synonyms:DR alpha chain|MHC cell surface glycoprotein|HLA class II histocompatibility antigen|MHC class II antigen DRA|histocompatibility antigen HLA-DR alpha
- Storage buffer:The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
- Storage temperature:Store in the dark at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent.
- Immunogen:thymocyte membrane
- Cat. no.:89298-884
- Supplier No.:GTX80021
Specifications
About this item
PerCP-conjugated Mouse Monoclonal antibody [MEM-12] to HLA-DR
Recommended Starting Dilutions:FACS: Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell lineIP: Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell lineWB: Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE Sample buffer. Do not heat/boil. Use under non-reducing conditionsOptimal dilutions should be determined experimentally by the researcher.
Type: Primary
Antigen: HLA-DR
Clonality: Monoclonal
Clone: MEM-12
Conjugation:
Epitope:
Host: Mouse
Isotype:
Reactivity: Human