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Specifications
- Antibody type:Primary
- Antigen name:Matrix Metalloproteinase 24
- Clonality:Polyclonal
- Gene ID:17391
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human,Mouse
- Antigen symbol:MMP24
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoPrecipitation:Yes
- Size:25μg
- Western blot:Yes
- Antigen synonyms:MMP25|MMP-24|MT-MMP 5|MT5MMP|MTMMP5|MT5-MMP|MT-MMP5
- Storage buffer:Phosphate-buffered saline, pH 7.4, containing 50% Glycerol and 0.1% Sodium azide
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Synthetic peptide corresponding to the propeptide region of mouse MMP-24.
- Purification:Immunogen affinity purified
- Cat. no.:89316-058
- Supplier No.:GTX11649
Specifications
About this item
ELISA (indirect): Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. IHC (frozen sections): Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1:2,000. This dilution is determined using a concentrated cell culture media from a stimulated cell line, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. The antibody identifies a band at 65 kD (zymogen) and also the activation and breakdown products. GTX11649 also recognizes non-reduced (native) MMP24. Note: Since cell types differ greatly in the quantity of MMP-24 produced, the conditioned media may require mitogen stimulation to visualize the bands by Western blotting. Treatment of cells with concanavolin-A or the phorbol ester TPA stimulates production of MMP24 in some cell types, and the enzyme can be recovered in cell lysates. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: MMP24
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse