About this item
Pellet Paint NF Co-Precipitant is a nonfluorescent dye-labeled carrier compatible with fluorescent sequencing.
- Efficient and rapid precipitation of sequencing products
- Efficient removal of dye terminators
- Direct visualization and tracking of precipitated material
- No effect on sequencing reaction
- Substitute for original Pellet Paint® Co-Precipitant for fluorescent detection applications
It facilitates rapid removal of BigDye Terminators during the alcohol precipitation of cycle sequencing reaction products
To avoid extra sample handling, this can be added directly to the reaction mix, template DNA, crude PCR samples, or dilution buffer before the cycle sequencing reaction. Although it absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible.
Cycle sequencing reactions can be precipitated rapidly with 1 µl of carrier per reaction and centrifugation times of 10 minutes. Resuspension of pelleted sequencing reaction products in deionized formamide can be confirmed by checking for dissolution of the carrier.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.