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Specifications
- Antibody type:Primary
- Antigen name:Peptidylprolyl isomerase
- Clonality:Polyclonal
- Host:Chicken
- Isotype:IgY
- Reactivity:Horse,Human,Bovine,Mouse,Cat
- Antigen symbol:Peptidylprolyl isomerase
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:50 µL
- Western blot:Yes
- Epitope:Multiple points of reactivity likely because of the polyclonal nature of the antibody
- Cross adsorption:No
- Format:Lyophilized
- Antigen synonyms:PPIase Pin-1|PIN1|Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1|Rotamase Pin1|Peptidyl-prolyl cis-trans isomerase Pin1
- Immunogen:Recombinant full length Peptidylprolyl isomerase (Pin1) purified from E.coli
- Cat. no.:10782-870
- Supplier No.:C-1398-50
Specifications
About this item
The enzyme Peptidylprolyl isomerase (Pin1) is responsible for flipping the proline ring from the cis to trans conformation. This enzyme regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity (ref: SWISSPROT). Pin1 is concentrated in the nucleus in small punctate structures and is particularly obvious in tumor cells.
The specificity of this antibody has been confirmed by WB. This antibody detects ~21 kDa Pin1 protein in whole HeLa cell homogenates. The antibody also properly detects Pin1 strongly as part of the nuclear matrix in HeLa cells with additional faint cytoplasmic staining.
Application Information:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Peptidylprolyl isomerase
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: Multiple points of reactivity likely because of the polyclonal nature of the antibody
Host: Chicken
Isotype: IgY
Reactivity: Human, Rat, Mouse,Bovine, Horse, Cat