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Specifications
- Antibody type:Primary
- Antigen name:Brain-derived Neurotrophic Factor
- Clonality:Polyclonal
- Host:Rabbit
- Isotype:IgG
- Reactivity:Rat,Mouse
- Antigen symbol:BDNF
- Conjugation:Unconjugated
- ELISA:Yes
- Size:100 µg
- Western blot:Yes
- Epitope:Multiple points of reactivity likely because of the polyclonal nature of the antibody
- Cross adsorption:No
- Format:Lyophilized
- Antigen synonyms:Brain-derived neurotrophic factor|Abrineurin
- Antibody fragment:IgG
- Storage buffer:Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.05% sodium azide.
- Storage temperature:Store antibody at 2-8°C protected from moisture. Divide antibody into useful aliquots and keep aliquots at –20 °C to –80 °C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
- Immunogen:Antibody was raised against rhBDNF as expressed in and purified from E. coli.
- Cat. no.:10782-580
- Supplier No.:R-1707-100
Specifications
About this item
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Western Blotting (denaturing and reducing): 0.2 to 1 µg/mL. Antibody detects 14 kDa mature BDNF monomer and 32 kDa proBDNF monomer in cell lysate and tissue homonenate. Antibody has only been tested on cell lysate and tissue homogenate of human origin. Acid-treated samples may give cleaner blots, and enhance signals for BDNF. R-1707-100 is not recommended for human serum samples. For human serum analysis, we recommend mouse monoclonal antibody to rhBDNF (M-1744-50/100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG).
Flow Cytometry: ~2 µg per 10^6 cells, methanol fixation. Note: R-1707-100 cannot be used to distinguish the flow cytometry signal originating from mature BDNF versus proBDNF.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: BDNF
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: Multiple points of reactivity likely because of the polyclonal nature of the antibody
Host: Rabbit
Isotype: IgG
Reactivity: Mouse, Rat