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Specifications
- Antibody type:Primary
- Antigen name:Microtubule-associated proteins 1A/1B light chain 3A
- Clonality:Polyclonal
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human,Rat
- Antigen symbol:MAP1LC3A
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:100 µL
- Western blot:Yes
- Epitope:N-terminal of Human MAP1LC3 A
- Cross adsorption:No
- Format:Lyophilized
- Antigen synonyms:MAP1BLC3Autophagy-related protein LC3 A|MAP1LC3|MAP1ALC3|LC3A|ATG8E|LC3
- Immunogen:A synthetic peptide (RSFADRCKEVQQI) corresponding to the N-terminal of human MAP1LC3 A protein conjugated to Blue Carrier Protein has been used as the immunogen. The sequence is homologous with mouse and rat MAP1LC3 A.
- Cat. no.:10782-478
- Supplier No.:R-146-100
Specifications
About this item
MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation). MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3). MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes.
IHC, WB and ELISA confirmed the specificity for MAP1LC3 A.
Application Information:
IHC, immunohistochemistry on 4% PFA fixed or 2% PLP fixation, 1:100-1000 primary antibody dilution. IC, Immunocytochemistry: acetone fixed specimens recommended. 1:100-1:1000 primary antibody dilution. Western Blot: R-146-100 requires samples to be denatured ONLY by boiling in SDS solution, not reduced. No signal is achieved using reduced samples. (Samples treated with DTT or Beta mercaptoethanol). 1:500 overnight 4 degrees is recommended for primary antibody dilution in western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: MAP1LC3A
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: N-terminal of Human MAP1LC3 A
Host: Rabbit
Isotype: IgG
Reactivity: Human, Rat