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Specifications
- Antibody type:Primary
- Antigen name:NeuN/Fox3
- Clonality:Monoclonal
- Clone:1B7
- Host:Mouse
- Isotype:IgG2a
- Reactivity:Pig,Human,Bovine,Rat,Mouse
- Antigen symbol:NeuN/Fox3
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- Size:100 µL
- Western blot:Yes
- Epitope:N-Terminal
- Cross adsorption:No
- Format:Lyophilized
- Antigen synonyms:Fox-1|Fox3|NeuN|Feminizing locus on X
- Immunogen:Antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli.
- Cat. no.:10782-120
- Supplier No.:M-377-100
Specifications
About this item
Fox3 is one of a family of mammalian homologues of Fox-1. The Fox proteins are about 46kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. Much interest has focused on Fox3 as a result of the recent finding that this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN/Fox-3 has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei and cytoplasm. Our antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.
The specificity of this antibody has been confirmed by WB. Two alternate transcripts can be seen at 46 kDa and 48 kDa.
Application Information:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500 - 1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: NeuN/Fox3
Clonality: Monoclonal
Clone: 1B7
Conjugation: Unconjugated
Epitope: N-terminal
Host: Mouse
Isotype: IgG2a
Reactivity: Human, Rat, Mouse, Bovine, Pig