TRIS Glycine Transfer Buffer, 10X (Transblot Buffer, 10X).

Tris Glycine Transfer Buffer, at a 1X concentration with the addition of 20% methanol, is used for electrophoretic transfer of proteins from denaturing polyacrylamide gel to a solid membrane – i.e. Western blotting or Tris-glycine gel electrophoresis.

Tris Glycine Transfer Buffer (10X) is prepared from molecular biology grade Tris base [tris(hydroxymethyl)- aminomethane] and glycine using Quality Biological’s Molecular Biology Grade (MBG) Water. The final product is sterile filtered using a 0.2 µm filter.