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Specifications
- Antibody type:Primary
- Antigen name:Actin Beta
- Clonality:Monoclonal
- Host:Mouse
- Isotype:IgM
- Reactivity:Zebrafish,Human,Rat,Mouse
- Antigen symbol:ACTB
- ELISA:Yes
- Size:150 µL
- Western blot:Yes
- Antigen synonyms:PS1TP5-binding protein 1|cytoplasmic 1|PS1TP5BP1|BRWS1|beta cytoskeletal actin|actin
- Storage buffer:PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.
- Shipping temperature:Store at -20°C
- Immunogen:Recombinant Protein
- Purification:Caprylic acid/ammonium sulfate precipitation
- Cat. no.:10097-424
- Supplier No.:HRP-60008
Specifications
About this item
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
Western Blot: HeLa Cells, 1:2000-1:20000
Type: Primary
Antigen: ACTB
Clonality: Monoclonal
Clone:
Conjugation:
Epitope:
Host: Mouse
Isotype: IgM
Reactivity: Human, Mouse, Rat, Zebrafish