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Specifications
- Antibody type:Primary
- Antigen name:ATP Synthase, H+ transporting, mitochondrial F1 Complex, alpha Subunit 1, cardiac muscle
- Clonality:Monoclonal
- Clone:1B10H3
- Host:Mouse
- Isotype:IgG2b
- Reactivity:Monkey,Human,Rat,Mouse
- Antigen symbol:ATP5A1
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:150 µL
- Western blot:Yes
- Antigen synonyms:ATP5A1
- Storage buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
- Shipping temperature:Store at -20°C
- Immunogen:Fusion Protein
- Purification:Protein A Purification
- Cat. no.:10083-332
- Supplier No.:66037-1-IG
Specifications
About this item
The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues.
IHC: Human Heart Tissue, 1:20-1:200; IF: Hela Cells, 1:20-1:200; Western Blot: Multi-Cells, 1:500-1:5000; FC: HeLa Cells, N/A
Type: Primary
Antigen: ATP5A1
Clonality: Monoclonal
Clone: 1B10H3
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Human, Mouse, Rat, Monkey