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Specifications
- Antibody type:Primary
- Antigen name:Green Fluorescence Protein
- Clonality:Monoclonal
- Clone:1E10H7
- Host:Mouse
- Isotype:IgG2a
- Reactivity:Aequorea victoria,Human
- Antigen symbol:GFP
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:150 µL
- Western blot:Yes
- Antigen synonyms:GFPuv|eGFP|Green Fluorescent Protein
- Storage buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
- Shipping temperature:Store at -20°C
- Immunogen:Recombinant Protein
- Purification:Protein A Purification
- Supplier No.:66002-1-IG
Specifications
About this item
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.
Western Blot: Recombinant Protein, 1:2000-1:20000; IP:Transfected HEK-293 Cells, 1:1000-1:10000; IF:Transfected HEK-293 Cells, 1:10-1:100
Type: Primary
Antigen: GFP tag
Clonality: Monoclonal
Clone: 1E10H7
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Aequorea victoria, Human