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Specifications
- Antibody type:Primary
- Antigen name:Microtubule-associated Protein 1 light chain 3 beta
- Clonality:Polyclonal
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Antigen symbol:LC3B
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:150 µL
- Western blot:Yes
- Antigen synonyms:MAP1LC3B|Autophagy-related protein LC3 B|MAP1 light chain 3-like protein 2|MAP1ALC3|MAP1A/MAP1B LC3 B|Microtubule-associated proteins 1A/1B light chain 3B [Precursor]|MAP1A/1B light chain 3 B|Autophagy-related ubiquitin-like modifier LC3 B
- Storage buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
- Shipping temperature:Store at -20°C
- Immunogen:Peptide
- Purification:Antigen affinity Purification
- Cat. no.:10089-932
- Supplier No.:18725-1-AP
Specifications
About this item
LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.
Western Blot: Human Brain Tissue, 1:500-1:5000; IHC: Human Heart Tissue, 1:20-1:200; IF:Chloroquine treated HepG2 Cells, 1:20-1:200; FC: HeLa Cells, N/A
Type: Primary
Antigen: LC3B
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat