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Specifications
- Antibody type:Primary
- Antigen name:CD27 molecule
- Clonality:Monoclonal
- Clone:O323
- Gene ID:939
- Host:Mouse
- Isotype:IgG1
- Neutralisation:Yes
- Reactivity:Baboon,Cynomolgus monkey,Macaque,Human
- Antigen symbol:CD27
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Size:100 µg
- Antigen synonyms:Tp55|S152|T14|TNFRSF7|CD27|S152. LPFS2
- Storage temperature:Store undiluted at 2-8°C, protect from light, do not freeze.
- Tested applications:Flow Cytometry
- Cat. no.:10051-672
- Supplier No.:70-0279-U100
Specifications
About this item
The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells.
The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD1d, IL-10 and TGF-beta (Kessel et al. 2012. Autoimm. Rev. 11(9): 670-677). The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27.
Type: Primary
Antigen: CD27
Clonality: Monoclonal
Clone: O323
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human, Baboon, Cynomolgus, Rhesus