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Specifications
- Description:PerfeCTa® PreAmp 5X SuperMix
- Size:40 rxn (of 50 µL rxn)
- Cat. no.:CA89409-170
- Supplier No.:95146-040
Specifications
About this item
PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing
- Unbiased, linear pre-amplification of up to 100 DNA targets
- 5X concentrated SuperMix maximizes sample input volume with diluted cDNA templates
- User-friendly inert AccuVue plate loading dye provides visual confirmation of reagent addition
A key component of PerfeCTa PreAmp SuperMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to pre-amplification.
PerfeCTa PreAmp SuperMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix will not affect product performance.
It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualize small reaction volumes and ensure accurate pipetting. PerfeCta® PreAmp SuperMix delivers unbiased pre-amplification of up to 100 target sequences from as little as 100 pg of total RNA. It is compatible with both TaqMan® 5’-nuclease probes or ds-DNA binding dye (i.e. SYBR® Green I) qPCR detection chemistries.