The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains (reviewed in 1). Alpha 4 (α4) chain associates with either beta 1 (β1) or beta 7 (β7) chain. It has been demonstrated that the putative ligand-binding sites of both integrin α4β1 and α4β7 is located on the α4 chain. These ligands included Madcam, VCAM, and fibronectin (2-4). Madcam is known as the principal ligand for integrin a4b7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin α4β7, the gut mucosal homing receptor for peripheral T cells (5). This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4α (IN1) (PSI 4827) and Integrin 4α (IN2) (PSI 4837), but not Integrin 4α (CT) (PSI 4783).

This recombinant protein can be used for WB, ELISA, MS and neutralization assays. For research use only.

100mM sodium phosphate, 500mM NaCl, 25 mM imidazole, 10% gycerol.

Store in working aliquots at -80°C. Avoid freeze/thaw cycles.