Nuclear factor kappa B (NF-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-B activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).

This recombinant protein can be used for WB, ELISA, MS and neutralization assays. For research use only.

100mM sodium phosphate, 10mM Tris, 500mM NaCl, 25 mM imidazole, 2mM MgCl2, 10% gycerol

Store in working aliquots at -80°C. Avoid freeze/thaw cycles.