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Specifications
- Antibody type:Primary
- Antigen name:chemokine (C-C motif) ligand 25
- Clonality:Polyclonal
- Gene ID:O15444
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:CCL25
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:CCL25
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK (human Thymus Expressed Chemokine).
- Purification:Anti-hTECK specific antibody was purified by affinity chromatography employing immobilized hTECK matrix.
- Cat. no.:10097-638
- Supplier No.:XP-5280
Specifications
About this item
This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0- 15.0 µg/mL of this antibody is required. ELISA: To detect hTECK by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTECK. Sandwich: To detect hTECK by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TECK (XP-5280Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hTECK specific antibody was purified by affinity chromatography employing immobilized hTECK matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: CCL25
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human