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Specifications
- Antibody type:Primary
- Antigen name:colony stimulating factor 1 (macrophage)
- Clonality:Polyclonal
- Gene ID:P09603
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:CSF1
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:CSF1
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF (human M-CSF).
- Purification:Anti-hM-CSF specific antibody was purified by affinity chromatography employing immobilized hM-CSF matrix.
- Cat. no.:10097-522
- Supplier No.:XP-5227
Specifications
About this item
Macrophage CSF, also known as CSF1, is produced by monocytes, fibroblasts and endothelial cells. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osetoclasts. Natural human MCSF is a dimeric glycoprotein of 70-90 kD molecular weight, existing in multiple glycosylation forms. It binds to a 165 kD glycoprotein of the receptor tyrosine kinase subclass III, a family that includes the receptors for platelet derived growth factor (PDGF) and stem cell factor (SCF).
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/mL), a concentration of 0.02 0.03 µg/mL of this antibody is required. ELISA: To detect hM-CSF by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. Sandwich: To detect hM-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human M-CSF (XP-5227Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hM-CSF specific antibody was purified by affinity chromatography employing immobilized hM-CSF matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: CSF1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human