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Specifications
- Antibody type:Primary
- Antigen name:chemokine (C-C motif) ligand 13
- Clonality:Polyclonal
- Gene ID:Q99616
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:CCL13
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:CCL13
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-4 (human Macrophage/Monocyte chemotactic protein-4).
- Purification:Anti-hMCP-4 specific antibody was purified by affinity chromatography employing immobilized hMCP-4 matrix.
- Cat. no.:10097-514
- Supplier No.:XP-5224
Specifications
About this item
Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-4 (30.00 ng/mL), a concentration of 3.0 - 4.0 µg/mL of this antibody is required. ELISA: To detect hMCP-4 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMCP-4. Sandwich: To detect hMCP-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MCP-4 (XP-5224Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4. Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-hMCP-4 specific antibody was purified by affinity chromatography employing immobilized hMCP-4 matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: CCL13
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human