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Specifications
- Antibody type:Primary
- Antigen name:interleukin 9
- Clonality:Polyclonal
- Gene ID:P15247
- Host:Rabbit
- Neutralisation:Yes
- Reactivity:Mouse
- Antigen symbol:Il9
- Conjugation:Unconjugated
- ELISA:Yes
- Size:0.1 mg
- Western blot:Yes
- Form:Lyophilized
- Antigen synonyms:Il9
- Shipping temperature:RT
- Immunogen:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-9 (murine IL-9).
- Purification:Anti-mIL-9 specific antibody was purified by affinity chromatography employing immobilized mIL-9 matrix.
- Cat. no.:10098-152
- Supplier No.:XP-5201
Specifications
About this item
IL-9 is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors; but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL-9 stimulates cell proliferation and prevents apoptosis. The IL-9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL-9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-9 (0.30 ng/mL), a concentration of 0.006 - 0.012 µg/mL of this antibody is required. ELISA: To detect mIL-9 by direct ELISA (using 100 µL/well antibody solution) a concentration of at least 0.5 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-9. Sandwich: To detect mIL-9 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-9 (XP-5201Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-9. Western Blot: To detect mIL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Anti-mIL-9 specific antibody was purified by affinity chromatography employing immobilized mIL-9 matrix.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: Il9
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Mouse